Brilliant Peel® Dual Dye

The non-toxic Dual Dye

Brilliant Peel® Dual Dye

Vial G-81025 Brilliant Peel® Dual Dye 0.5 ml
Vial, 5 pcs. per box, sterile
Syringe G-81015 Brilliant Peel® Dual Dye 0.5 ml
Syringe, 5 pcs. per box, sterile

  • Intense and selective staining of ILM, ERM and vitreous remnants
  • Fast sinking – maximized contact surface with tissue due to higher density
  • Safe application under air and BSS
  • Quick and easy application (Ready to use)
  • Physiological osmolarity
  • Biocompatible


“Excellent staining of pre-retinal membranes and vitreous remnants.”
Senior Consultant Jürgen Steinhauer MD University Eye Clinic Witten/Herdecke, County Hospital Hagen, St.-Josefs-Hospital, Germany

“Outstanding staining properties and an impressing sinking behavior makes Brilliant Peel Dual Dye the perfect tool for a save peeling in epiretinal macular procedures. Flawless for a fast and reliable multiple staining of different membrane parts.”
Prof. Dr. Lars-Olof Hattenbach Director of Eye Clinic Ludwigshafen, Germany

“Even under yellow UV-IOL the shape of the retinal nerve fiber layer (RNFL) on the ILM was perfectly visible. A highly promising new dye with excellent sinking properties.”
A. Viestenz MD University Clinic of Saarland, Homburg, Germany

Composition and properties of Brilliant Peel®

Composition of one 0.5 ml syringe / vial:
0.125 mg Brilliant Blue G,
0.65 mg Bromphenol Blue,
0.1 ml D2O
0.95 mg Na2HPO4 x 2 H2O
0.15 mg NaH2PO4 x 2 H2O
4.1 mg NaCl
ad 0.5 ml water for injection purposes
0.25 g/l Brilliant Blue G
1.3 g/l Bromphenol Blue
1.03 g/cm3

Fields of application

Brilliant Peel® Dual Dye was developed for specific staining of the inner limiting membrane (ILM) and epiretinal membrane (ERM). Specific staining of the ILM and ERM allows them to be clearly distinguished from the underlying retinal tissue, thus making the challenging surgical removal of the ILM and ERM easier and safer. Due to the density of 1.03 g/cm3 Brilliant Peel® Dual Dye quickly sinks to the fundus of the eye without diffuse dispersion in the whole bulbus. Brilliant Peel® Dual Dye can, to some extent, also be used for staining vitreous remnants.

Characteristics Brilliant Peel® Other Dyes
BBG ICG TB Acid Violet-17 Lutein
Chemical Classification Triphenylmethane Cyanine Diazo Triphenylmethane Carotinoide
Color Blue Green Blue Violet Yellow-Orange
Dye 12 Brillant Blau G Indocyanine Green Trypan Blue Acid Violet-17 Lutein
Toxicity 1, 2, 3, 6, 7, 9, 10, 12, 13 no yes moderate moderate no
Approval yes no yes yes yes
Affinity for ILM 4, 5, 8, 14, 15 high high low high low
Affinity for ERM 2, 14 niedrig low high low n.a.
Affinity for vitreous body 11, 14, 15 low low low low high
Exporsure time short short long short short
Liquid/Gas exchange no no yes no no

1 Lüke C, et al.: Retinal tolerance to dyes, Br J Ophthalmol, 2005, 89, 1188-1191
2 Haritoglou C, et al.: Färbetechniken in der Makulachirurgie, Ophthalmologe, 2006, 103, 927-934
3 Ueno A, et al.: Biocompatibility of Brilliant Blue G in a rat model of subretinal injection, Retina, 2007, 27, 499-504
4 Enaida H, et al.: Brilliant Blue G selectively stains the internal limiting membrane – Brilliant Blue G assisted membrane peeling, Retina, 2006, 26, 631 – 636
5 Enaida H, et al.: Preclinical investigation of internal limiting membrane staining and peeling using intravitreal Brilliant Blue G, Retina, 2006, 26, 623-630
6 Hisatomi T, et al.: Staining ability and biocompatibility of Brilliant Blue G – preclinical study of Brilliant Blue G as an adjunct for capsular staining, Arch Ophthalmol, 2006, 124, 514-519
7 Goldman JM, et al.: Adjunct devices for managing challenging cases in cataract surgery – capsular staining and ophthalmic viscosurgical devices, Curr Opin Ophthalmol, 2007, 18, 52-57
8 Meyer CH, et al.: Historical considerations in applying vital dyes in vitreoretinal surgery: from early experiments to advanced chromovitrectomy, Expert Rev. Ophthalmol., 2007, 71-77
9 Hiebl W, et al.: Substances for staining biological tissues: use of dyes in ophthalmology, Klin Monatsbl Augenh, 2005, 222, 309-311
10 Frank Schuettauf, Christos Haritoglou, Christian A. May, Robert Rejdak, Anna Mankowska, Wolfgang Freyer, Kirsten Eibl, Eberhart Zrenner, Anselm Kampik and Sebastian Thaler, Administration of Novel Dyes for Intraocular Surgery: An In Vivo Toxicity Animal Study, Invest Ophthalmol Vis Sci. 2006; 47:3573–3578
11 Christos Haritoglou, Ricarda G Schumann, Rupert Strauss, Siegfried G Priglinger, Aljoscha S Neubauer, Anselm Kampik, Vitreoretinal surgery using bromphenol blue as a vital stain: evaluation of staining characteristics in humans, Br J Ophthalmol 2007; 91:1125–1128
12 Rodrigues EB, et al.: Vital dyes for chromovitrectomy, Curr Opin Ophthalmol, 2007 May; 18(3):179-87
13 Furlani BA, et al.: Lutein and zeaxanthin toxicity with and without brilliant blue in rabbits. J Ocul Pharmacol Ther. 2014 Sep; 30(7):559-66. doi: 10.1089/jop.2013.0171. Epub 2014 Jun 5.
14 Tura A, et al.: Testing the effects of the dye acid violet-17 on retinal function for an intraocular application in vitreo-retinal surgery. Graefes Arch Clin Exp Opthalmol. 2014 Dec; 252(12):1927-37. doi: 10.1007/s00417-014-2761-9. Epub 2014 Sep 14.
15 Patent DE102012103097 A1
16 Sousa-Martins D, et al.: Use of lutein and zeaxanthin alone or combined with Brilliant Blue to identify intraocular structures intraoperatively. Retina. 2012 Jul; 32(7):1328-36. doi: 10.1097/IAE.0b013e318239e2b6.